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| 货号 | EK1984 |
|---|---|
| 产品名称 | Mouse CRELD2 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of mouse CRELD2 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, S23-L350 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | Creld2 |
| 基因全名 | cysteine-rich with EGF-like domains 2 |
| Uniprot ID | Q9CYA0 |
| 指标别名 | Cysteine-rich with EGF-like domain protein 2; Creld2 |
| 背景资料 | Cysteine-rich with EGF-like domain protein 2 is a protein that in humans is encoded by the CRELD2 gene. It is mapped to 15; 15 E3. CRELD2 was identified by searching a sequence database using CRELD1 as the query. Like CRELD1, with which it shares 38% amino acid identity, the deduced 321-amino acid CRELD2 protein contains EGF-like repeats and a highly conserved domain rich in glutamic acid and tryptophan (WE domain). The major distinction between CRELD1 and CRELD2 is that CRELD1 has 2 type III transmembrane domains in its C terminus, while CRELD2 has none. CRELD2 shares 67% and 69% amino acid identity with its Chinese hamster and mouse homologs, respectively. |
| 显色时间 | 20-25 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1984-CAP | 96-well plate precoated with anti- mouse CRELD2 antibody | 1 |
| EK1984-ST | Lyophilized recombinant mouse CRELD2 standard | 10ng/tube×2 |
| EK1984-DA | Biotinylated anti- mouse CRELD2 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 214 | 1244 | 5866 | 219 | 1247 | 6512 |
| Standard deviation | 10.06 | 65.93 | 328.50 | 14.67 | 63.60 | 312.58 |
| CV(%) | 4.7% | 5.3% | 5.6% | 6.7% | 5.1% | 4.8% |
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.042 | 0.091 | 0.137 | 0.220 | 0.404 | 0.671 | 1.218 | 2.080 |
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