货号 | EK2010 |
---|---|
产品名称 | Mouse CHI3L3 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse CHI3L3 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, Y22-Y398 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | Chil3 |
基因全名 | chitinase-like 3 |
Uniprot ID | O35744 |
指标别名 | Chitinase-like protein 3; Beta-N-acetylhexosaminidase Ym1; Chitinase-3-like protein 3; ECF-L; Eosinophil chemotactic cytokine; Secreted protein Ym1; Chil3; Chi3l3; Ym1 |
背景资料 | Chitinase 3-like 3, also known as ECF-L and Ym1, is a secreted 377 amino acid lectin that binds heparin and GlcN oligomers. This gene is mapped to 3 F2.2; 3 46.49 Cm. CHI3L3 is produced primarily by macrophages during inflammation. ECF-L shares homology with chitinase family proteins, but lacks enzymatic activity. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2010-CAP | 96-well plate precoated with anti- mouse CHI3L3 antibody | 1 |
EK2010-ST | Lyophilized recombinant mouse CHI3L3 standard | 20ng/tube×2 |
EK2010-DA | Biotinylated anti- mouse CHI3L3 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 474 | 1746 | 8158 | 552 | 1822 | 8247 |
Standard deviation | 24.65 | 92.54 | 448.69 | 33.67 | 116.61 | 552.55 |
CV(%) | 5.2% | 5.3% | 5.5% | 6.1% | 6.4% | 6.7% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.030 | 0.076 | 0.123 | 0.221 | 0.403 | 0.754 | 1.313 | 2.035 |
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