货号 | EK1815 |
---|---|
产品名称 | Mouse CES3 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse CES3 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, Y19-L565 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | Ces1d |
基因全名 | carboxylesterase 1D |
Uniprot ID | Q8VCT4 |
指标别名 | Carboxylesterase 1D; Carboxylesterase 3; Fatty acid ethyl ester synthase; FAEE synthase; Triacylglycerol hydrolase; TGH; Ces1d; Ces1; Ces3 |
背景资料 | This gene is mapped to 8; 8 C5. It encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. Enzyme that hydrolizes long-chain fatty acids and thioesters; may play a role in lipid metabolism and/or the elimination of toxic substances. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This gene is expressed in several tissues, particularly in colon, trachea and in brain, and the protein participates in colon and neural drug metabolism. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported, but the biological validity and/or full-length nature of some variants have not been determined. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1815-CAP | 96-well plate precoated with anti- mouse CES3 antibody | 1 |
EK1815-ST | Lyophilized recombinant mouse CES3 standard | 10ng/tube×2 |
EK1815-DA | Biotinylated anti- mouse CES3 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 257 | 1095 | 4121 | 303 | 1263 | 4314 |
Standard deviation | 15.93 | 71.2 | 177.2 | 15.76 | 77.04 | 310.6 |
CV(%) | 6.2% | 6.5% | 4.3% | 5.2% | 6.1% | 7.2% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.018 | 0.170 | 0.291 | 0.609 | 1.135 | 1.763 | 2.142 | 2.383 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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