货号 | EK2089 |
---|---|
产品名称 | Mouse CD99 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse CD99 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | E.coli, D29-G137 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 125pg/ml-8000pg/ml |
基因名称 | Cd99 |
基因全名 | CD99 antigen |
Uniprot ID | Q8VCN6 |
指标别名 | CD99 antigen; Paired immunoglobin-like type 2 receptor-ligand; PILR-L; CD99; Cd99; Pilrl |
背景资料 | CD99 antigen (Cluster of differentiation 99), also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein that is encoded by the CD99 gene in humans. It is mapped to 4; 4. The protein has a mass of 32 kD. Unusually for a gene present on the X chromosome, the CD99 gene does not undergo X inactivation, and it was the first such pseudoautosomal gene to be discovered in humans. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2089-CAP | 96-well plate precoated with anti- mouse CD99 antibody | 1 |
EK2089-ST | Lyophilized recombinant mouse CD99 standard | 10ng/tube×2 |
EK2089-DA | Biotinylated anti- mouse CD99 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 153 | 549 | 2842 | 214 | 654 | 3861 |
Standard deviation | 6.27 | 23.61 | 130.73 | 10.91 | 38.59 | 258.69 |
CV(%) | 4.1% | 4.3% | 4.6% | 5.1% | 5.9% | 6.7% |
Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
O.D. | 0.110 | 0.219 | 0.378 | 0.541 | 0.885 | 1.190 | 1.787 | 2.465 |
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