货号 | EK2053 |
---|---|
产品名称 | Mouse CD69 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse CD69 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, N62-R199 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <20pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Cd69 |
基因全名 | CD69 antigen |
Uniprot ID | P37217 |
指标别名 | Early activation antigen CD69; CD69; Cd69 |
背景资料 | CD69 (Cluster of Differentiation 69) is a human transmembrane C-Type lectin protein encoded by the CD69 gene. This gene is mapped to 12p13.31. This gene encodes a member of the calcium dependent lectin superfamily of type II transmembrane receptors. Expression of the encoded protein is induced upon activation of T lymphocytes, and may play a role in proliferation. Furthermore, the protein may act to transmit signals in natural killer cells and platelets. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2053-CAP | 96-well plate precoated with anti- mouse CD69 antibody | 1 |
EK2053-ST | Lyophilized recombinant mouse CD69 standard | 10ng/tube×2 |
EK2053-DA | Biotinylated anti- mouse CD69 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 24 | 63 | 287 | 27 | 84 | 429 |
Standard deviation | 1.08 | 3.34 | 13.78 | 1.65 | 5.38 | 31.32 |
CV(%) | 4.5% | 5.3% | 4.8% | 6.1% | 6.4% | 7.3% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.031 | 0.129 | 0.217 | 0.389 | 0.759 | 1.223 | 2.057 | 2.432 |
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