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| 货号 | EK2013 |
|---|---|
| 产品名称 | Mouse CD39/ENTPD1 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of mouse CD39 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, T38-I478 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | Entpd1 |
| 基因全名 | ectonucleoside triphosphate diphosphohydrolase 1 |
| Uniprot ID | P55772 |
| 指标别名 | Ectonucleoside triphosphate diphosphohydrolase 1; NTPDase 1; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Lymphoid cell activation antigen; CD39; Entpd1; Cd39 |
| 背景资料 | Ectonucleoside triphosphate diphosphohydrolase-1 (gene: ENTPD1; protein: NTPDase1) also known as CD39 (Cluster of Differentiation 39), is a typical cell surface-located enzymes with an extracellularly facing catalytic site. This gene is mapped to 19 C3; 19 34.25 cM. NTPDase1 is an ectonucleotidase that catalyse the hydrolysis of γ- and β-phosphate residues of triphospho- and diphosphonucleosides to the monophosphonucleoside derivative. For example, NTPDase1 hydrolyzes P2 receptor ligands, namely ATP, ADP, UTP and UDP with similar efficacy. NTPDase1 can therefore affect P2 receptor activation and functions. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2013-CAP | 96-well plate precoated with anti- mouse CD39/ENTPD1 antibody | 1 |
| EK2013-ST | Lyophilized recombinant mouse CD39/ENTPD1 standard | 10ng/tube×2 |
| EK2013-DA | Biotinylated anti- mouse CD39/ENTPD1 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 59 | 215 | 956 | 60 | 234 | 991 |
| Standard deviation | 3.01 | 11.40 | 53.54 | 3.96 | 15.91 | 70.36 |
| CV(%) | 5.1% | 5.3% | 5.6% | 6.6% | 6.8% | 7.1% |
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.087 | 0.200 | 0.279 | 0.467 | 0.752 | 1.205 | 1.958 | 2.304 |
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