货号 | EK1958 |
---|---|
产品名称 | Mouse CD200R1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse CD200R1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, T26-P238 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 78pg/ml-5000pg/ml |
基因名称 | Cd200r1 |
基因全名 | CD200 receptor 1 |
Uniprot ID | Q9ES57 |
指标别名 | Cell surface glycoprotein CD200 receptor 1; CD200 cell surface glycoprotein receptor; Cell surface glycoprotein OX2 receptor 1; Cd200r1; Mox2r; Ox2r |
背景资料 | Cell surface trasmembrane glycoprotein CD200 receptor 1 is a protein that in humans is encoded by the CD200R1 gene. It is mapped to 16; 16 B4. CD200R1 is expressed on the surface of myeloid cells and CD4+ T cells. It interacts with CD200 transmembrane glycoprotein that can be expressed on variety of cells including neurons, epithelial cells, endothelial cells, fibroblasts, and lymphoid cells CD200R1 activation regulates the expression of pro-inflammatory molecules such as tumor necrosis factor (TNF-alpha), interferons, and inducible nitric oxide synthase. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1958-CAP | 96-well plate precoated with anti- mouse CD200R1 antibody | 1 |
EK1958-ST | Lyophilized recombinant mouse CD200R1 standard | 10ng/tube×2 |
EK1958-DA | Biotinylated anti- mouse CD200R1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 128 | 681 | 2537 | 139 | 708 | 3076 |
Standard deviation | 5.76 | 44.27 | 169.98 | 9.45 | 41.77 | 187.64 |
CV(%) | 4.5% | 6.5% | 6.7% | 6.8% | 5.9% | 6.1% |
Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1250 | 2500 | 5000 |
O.D. | 0.073 | 0.378 | 0.470 | 0.614 | 0.821 | 1.124 | 1.415 | 1.809 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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