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Mouse Cadherin-17 PicoKine ELISA Kit 96T

价:
2800.00
价:
¥2800.00

号:EK1998

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK1998
    产品名称 Mouse Cadherin-17 PicoKine ELISA Kit
    产品描述 For quantitative detection of mouse Cadherin-17 in cell culture supernates, serum and plasma (heparin).
    免疫原 NSO, F26-S827
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <10pg/ml
    检测范围 46.9pg/ml-3000pg/ml
    基因名称 Cdh17
    基因全名 cadherin 17
    Uniprot ID Q9R100
    指标别名 Cadherin-17; BILL-cadherin; Liver-intestine cadherin; LI-cadherin; P130; Cdh17
    背景资料 Cadherin-17 is a protein that in humans is encoded by the CDH17 gene. It is mapped to 4; 4 A1. This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. The encoded protein is cadherin-like, consisting of an extracellular region, containing 7 cadherin domains, and a transmembrane region but lacking the conserved cytoplasmic domain. The protein is a component of the gastrointestinal tract and pancreatic ducts, acting as an intestinal proton-dependent peptide transporter in the first step in oral absorption of many medically important peptide-based drugs. The protein may also play a role in the morphological organization of liver and intestine.
    显色时间 15-20

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK1998-CAP 96-well plate precoated with anti- mouse Cadherin-17 antibody 1
    EK1998-ST Lyophilized recombinant mouse Cadherin-17 standard 3ng/tube×2
    EK1998-DA Biotinylated anti- mouse Cadherin-17 antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30 ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 90 369 1459 91 370 1485
    Standard deviation 4.41 19.56 97.75 5.82 21.46 117.32
    CV(%) 4.9% 5.3% 6.7% 6.4% 5.8% 7.9%

    Mouse Cadherin-17 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000
    O.D. 0.058 0.151 0.213 0.335 0.625 0.949 1.507 2.384
    *典型数据仅供参考。用户需要自己建立标准曲线。

Instructions

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