货号 | EK1833 |
---|---|
产品名称 | Mouse CA12 PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse CA12 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, A25-S301 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | Ca12 |
基因全名 | carbonic anhydrase 12 |
Uniprot ID | Q8CI85 |
指标别名 | Carbonic anhydrase 12 (EC:4.2.1.1); Carbonate dehydratase XII; Carbonic anhydrase XII; CA-XII; Ca12; Car12 |
背景资料 | Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. It is mapped to 9; 9 C. This gene encodes a membrane-bound member of the alpha carbonic anhydrase family of enzymes that catalyze the reversible hydration of carbon dioxide to bicarbonate. These proteins participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Alternative splicing results in multiple transcript variants. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1833-CAP | 96-well plate precoated with anti- mouse CA12 antibody | 1 |
EK1833-ST | Lyophilized recombinant mouse CA12 standard | 10ng/tube×2 |
EK1833-DA | Biotinylated anti- mouse CA12 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 24 | 105 | 321 | 28 | 111 | 358 |
Standard deviation | 1.08 | 5.99 | 14.77 | 1.43 | 6.55 | 26.49 |
CV(%) | 4.5% | 5.7% | 4.6% | 5.1% | 5.9% | 7.4% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.010 | 0.031 | 0.064 | 0.137 | 0.376 | 0.922 | 1.879 | 2.599 |
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