货号 | EK1955 |
---|---|
产品名称 | Mouse C1R PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse C1R in cell culture supernates, serum and plasma (heparin, EDTA, citrate) and urine. |
免疫原 | NSO, S18-D705 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.1ng/ml |
检测范围 | 0.625ng/ml-40ng/ml |
基因名称 | C1ra; C1rb |
基因全名 | complement component 1, r subcomponent A; complement component 1, r subcomponent B |
Uniprot ID | Q8CG16; Q8CFG9 |
指标别名 | Complement C1r-A subcomponent; Complement component 1 subcomponent r-A; C1ra; C1r; Complement C1r-B subcomponent; Complement component 1 subcomponent r-B; C1rb |
背景资料 | Complement C1r subcomponent is a protein involved in the complement system of the innate immune system. In humans, C1r is encoded by the C1R gene. It is mapped to 6; 6 F2 and 6 F2; 6. C1r has been shown to interact with C1s. C1r cleaves C1s to form the active form of C1s. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1955-CAP | 96-well plate precoated with anti- mouse C1R antibody | 1 |
EK1955-ST | Lyophilized recombinant mouse C1R standard | 40ng/tube×2 |
EK1955-DA | Biotinylated anti- mouse C1R antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1004 | 3663 | 23406 | 1174 | 4272 | 24979 |
Standard deviation | 47.19 | 197.80 | 1451.17 | 79.83 | 196.51 | 1448.78 |
CV(%) | 4.7% | 5.4% | 6.2% | 6.8% | 4.6% | 5.8% |
Concentration(pg/ml) | 0 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 | 40000 |
O.D. | 0.040 | 0.145 | 0.248 | 0.437 | 0.769 | 1.189 | 1.881 | 2.337 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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