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| 货号 | EK1809 |
|---|---|
| 产品名称 | Mouse BMP-10 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of mouse BMP-10 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, N314-R421 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 15.6pg/ml-1000pg/ml |
| 基因名称 | Bmp10 |
| 基因全名 | bone morphogenetic protein 10 |
| Uniprot ID | Q9R229 |
| 指标别名 | Bone morphogenetic protein 10; BMP-10; BMP10 |
| 背景资料 | Bone morphogenetic proteins (BMPs) constitute a subfamily within the TGF-β superfamily of structurally related signaling proteins. This gene is mapped to 6; 6 D1. Members of this superfamily are widely distributed throughout the body and are involved in diverse physiological processes during both pre- and postnatal life. BMP-10 plays a crucial role in the development of the embryonic heart by acting to stimulate and maintain cardiomyocyte proliferation. It can signal through various receptor complexes usually containing BMPR-1A, BMPR-1B, ALK1, ALK3, or ALK6. The interaction of BMP-10 with its specific receptors can induce signaling initiated by the phosphorylation of SMAD transcription factors, including SMAD1, SMAD5, or SMAD8, but can also induce SMAD independent processes. BMP-10 is structurally related to BMP-9, and both can inhibit endothelial cell proliferation and migration. Recombinant human BMP-10 is a 24.4 kDa homodimeric disulfide-linked protein consisting of two 108 amino acid subunits, which correspond to amino acid residues 317 to 424 of the full-length BMP-10 precursor. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1809-CAP | 96-well plate precoated with anti- human mouse BMP antibody | 1 |
| EK1809-ST | Lyophilized recombinant human mouse BMP standard | 10ng/tube×2 |
| EK1809-DA | Biotinylated anti- human mouse BMP antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 29 | 211 | 444 | 31 | 226 | 487 |
| Standard deviation | 1.19 | 9.5 | 27.53 | 1.71 | 13.1 | 37.01 |
| CV(%) | 4.1% | 4.5% | 6.2% | 5.5% | 5.8% | 7.6% |
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.003 | 0.085 | 0.158 | 0.328 | 0.591 | 1.152 | 1.943 | 2.722 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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