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Mouse ANGPTL7 PicoKine ELISA Kit 96T

价:
2800.00
价:
¥2800.00

号:EK2036

牌:BOSTER 博士德

账期 货到付款

EA (预计5-7工作日到货)

工作时间

周一至周五:9:00-18:00

咨询电话

0771-3293894

在线咨询

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Product Brief

  • 产品概况

    货号 EK2036
    产品名称 Mouse ANGPTL7 PicoKine ELISA Kit
    产品描述 For quantitative detection of mouse ANGPTL7 in cell culture supernates, serum and plasma (heparin, EDTA).
    免疫原 CHO, Q22-P337
    交叉反应 There is no detectable cross-reactivity with other relevant proteins.
    敏感性 <50pg/ml
    检测范围 93.7pg/ml-6000pg/ml
    基因名称 Angptl7
    基因全名 angiopoietin-like 7
    Uniprot ID Q8R1Q3
    指标别名 Angiopoietin-related protein 7; Angiopoietin-like protein 7; Angptl7
    背景资料 Angiopoietin-related protein 7 is a protein that in humans is encoded by the ANGPTL7 gene. This gene is mapped to 4; 4 E2. Angiopoietin-like Protein 7 (ANGPTL7), also known as cornea-derived transcript 6 (CDT6), is a secreted 45 kDa protein that contains a coiled-coil domain (aa 30‑110) and a fibrinogen-like domain (aa 113‑334). ANGPTL7 is expressed in the stromal layer of the cornea, and it forms disulfide-linked homotetramers. It contributes to corneal morphogenesis by suppressing angiogenesis and promoting the deposition of extracellular matrix components. ANGPTL7 is up-regulated in glaucoma and accumulates in the aqueous humor. Mature mouse ANGPTL7 shares 88% and 98% sequence identity with human and rat ANGPTL7.
    显色时间 15-20

    试剂盒内容

    SKU 名称 数量
    Catalog number Description Quantity
    EK2036-CAP 96-well plate precoated with anti- mouse ANGPTL7 antibody 1
    EK2036-ST Lyophilized recombinant mouse ANGPTL7 standard 10ng/tube×2
    EK2036-DA Biotinylated anti- mouse ANGPTL7 antibody 130μl(dilution 1:100)
    AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 130μl(dilution 1:100)
    AR1106-1 Sample diluent buffer 30ml
    AR1106-2 Antibody diluent buffer 12ml
    AR1106-3 ABC diluent buffer 12ml
    AR1104 TMB color developing agent 10ml
    AR1105 TMB stop solution 10ml
    PLA-SEA Adhesive cover 4
    *小贴士:额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

    需要用户自己提供的器材和试剂

    • 1.96孔读板仪器。
    • 2.自动洗板机.
    • 3.移液器和枪头。建议使用多通道移液器/排枪。
    • 4.Eppendorf 管。
    • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
      • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
      • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    *样品准备的相关试剂也不含在试剂盒中。

    批内差/批间差

    Intra-Assay Precision Inter-Assay Precision
    Sample 1 2 3 1 2 3
    n 16 16 16 24 24 24
    Mean(pg/ml) 179 600 2224 182 624 2685
    Standard deviation 7.70 27.00 104.53 10.19 36.19 166.47
    CV(%) 4.3% 4.5% 4.7% 5.6% 5.8% 6.2%

    Mouse ANGPTL7 PicoKine ELISA Kit的典型数据示例(取自最近的一批次质检数据)

    (TMB 孵育条件: 37°C, 15-20)
    Concentration(pg/ml) 0 93.7 187.5 375 750 1500 3000 6000
    O.D. 0.044 0.109 0.162 0.268 0.480 0.828 1.459 2.148
    *典型数据仅供参考。用户需要自己建立标准曲线。

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