货号 | EK1661 |
---|---|
产品名称 | Mouse AFP PicoKine ELISA Kit |
产品描述 | For quantitative detection of mouse AFP in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, K19-V605 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | Afp |
基因全名 | alpha fetoprotein |
Uniprot ID | P02772 |
指标别名 | Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; Afp |
背景资料 | alpha-Fetoprotein (AFP), is albuminoid superfamily protein that is synthesized in the fetus primarily by the liver, yolk sac, and tissues of gastrointestinal origin. This gene is mapped to 5 E1; 5 44.77 cM. AFP acts as a carrier protein for steroids, bilirubin, fatty acids, retinoids, and flavonoids. In addition, it can exert immunosuppressive activity, regulate cell proliferation and apoptosis, initiate intracellular signaling, and contribute to cell invasion. Altered levels of both fetal and maternal AFP have been associated with hypothyroidism, autoimmune disorders, and heart defects. Low maternal serum AFP levels are associated with a higher incidence of Down syndrome, whereas higher levels are associated with spina bifida and anencephaly. Elevated AFP levels are also coincident with liver, stomach, and germ cell cancers. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1661-CAP | 96-well plate precoated with anti- mouse AFP antibody | 1 |
EK1661-ST | Lyophilized recombinant mouse AFP standard | 10ng/tube×2 |
EK1661-DA | Biotinylated anti- mouse AFP antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 50 | 189 | 834 | 57 | 204 | 928 |
Standard deviation | 2.40 | 9.26 | 41.70 | 2.91 | 12.04 | 59.39 |
CV(%) | 4.8% | 4.9% | 5.0% | 5.1% | 5.9% | 6.4% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.006 | 0.065 | 0.134 | 0.269 | 0.466 | 0.924 | 1.552 | 2.366 |
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