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| 货号 | EK1690 |
|---|---|
| 产品名称 | Human Uromodulin/umod PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human Uromodulin in cell culture supernates, serum and plasma (heparin). |
| 免疫原 | NSO,D25-F587 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | UMOD |
| 基因全名 | uromodulin |
| Uniprot ID | P07911 |
| 指标别名 | Uromodulin; Tamm-Horsfall urinary glycoprotein; THP; Uromodulin, secreted form; UMOD |
| 背景资料 | The Tamm–Horsfall glycoprotein (THP), also known as uromodulin, is a glycoprotein that in humans is encoded by the UMOD gene. The protein encoded by this gene is the most abundant protein in mammalian urine under physiological conditions. Its excretion in urine follows proteolytic cleavage of the ectodomain of its glycosyl phosphatidylinosital-anchored counterpart that is situated on the luminal cell surface of the loop of Henle. This protein may act as a constitutive inhibitor of calcium crystallization in renal fluids. Excretion of this protein in urine may provide defense against urinary tract infections caused by uropathogenic bacteria. Defects in this gene are associated with the renal disorders medullary cystic kidney disease-2 (MCKD2), glomerulocystic kidney disease with hyperuricemia and isosthenuria (GCKDHI), and familial juvenile hyperuricemic nephropathy (FJHN). Alternative splicing of this gene results in multiple transcript variants. |
| 显色时间 | 20-25 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1690-CAP | 96-well plate precoated with anti- human Uromodulin antibody | 1 |
| EK1690-ST | Lyophilized recombinant human Uromodulin standard | 10ng/tubex2 |
| EK1690-DA | Biotinylated anti- human Uromodulin antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 215 | 1090 | 5159 | 226 | 982 | 5437 |
| Standard deviation | 13.33 | 57.77 | 211.51 | 16.72 | 62.84 | 293.59 |
| CV(%) | 6.2% | 5.3% | 4.1% | 7.4% | 6.4% | 5.4% |
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.124 | 0.166 | 0.196 | 0.291 | 0.436 | 0.676 | 1.158 | 2.017 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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