货号 | EK1678 |
---|---|
产品名称 | Human TREM2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human TREM2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,H19-E167 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | TREM2 |
基因全名 | triggering receptor expressed on myeloid cells 2 |
Uniprot ID | Q9NZC2 |
指标别名 | Triggering receptor expressed on myeloid cells 2; TREM-2; Triggering receptor expressed on monocytes 2; TREM2 |
背景资料 | Triggering receptor expressed on myeloid cells 2(TRM2) encodes a membrane protein that forms a receptor signaling complex with the TYRO protein tyrosine kinase binding protein. The encoded protein functions in immune response and may be involved in chronic inflammation by triggering the production of constitutive inflammatory cytokines. Defects in this gene are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL). Alternative splicing results in multiple transcript variants encoding different isoforms. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1678-CAP | 96-well plate precoated with anti- human TREM2 antibody | 1 |
EK1678-ST | Lyophilized recombinant human TREM2 standard | 10ng/tubex2 |
EK1678-DA | Biotinylated anti- human TREM2 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 72 | 232 | 780 | 74 | 222 | 829 |
Standard deviation | 2.95 | 9.74 | 37.44 | 3.1 | 10.65 | 55.54 |
CV(%) | 4.1% | 4.2% | 4.8% | 4.2% | 4.8% | 6.7% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.011 | 0.093 | 0.232 | 0.425 | 0.719 | 1.541 | 1.872 | 2.587 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894