货号 | EK1684 |
---|---|
产品名称 | Human Transthyretin/TTR PicoKine ELISA Kit |
产品描述 | For quantitative detection of human TTR in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli,G21-E147 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | TTR |
基因全名 | transthyretin |
Uniprot ID | P02766 |
指标别名 | Transthyretin; ATTR; Prealbumin; TBPA; TTR; PALB |
背景资料 | Transthyretin (TTR) is also known as prealbumin. It is mapped to chromosome region 18q11.2-q12.1. This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1684-CAP | 96-well plate precoated with anti- Human TTR antibody | 1 |
EK1684-ST | Lyophilized recombinant Human TTR standard | 100ng/tubex2 |
EK1684-DA | Biotinylated anti- Human TTR antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 778 | 2764 | 9900 | 774 | 2895 | 9099 |
Standard deviation | 37.34 | 110.56 | 712.8 | 39.47 | 167.91 | 718.82 |
CV(%) | 4.8% | 4% | 7.2% | 5.1% | 5.8% | 7.9% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.050 | 0.083 | 0.107 | 0.135 | 0.232 | 0.505 | 1.079 | 2.263 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894