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| 货号 | EK2042 |
|---|---|
| 产品名称 | Human TPP1 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human TPP1 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, L196-P563 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | TPP1 |
| 基因全名 | tripeptidyl peptidase 1 |
| Uniprot ID | O14773 |
| 指标别名 | Tripeptidyl-peptidase 1; TPP-1; Cell growth-inhibiting gene 1 protein; Lysosomal pepstatin-insensitive protease; LPIC; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase I; TPP-I; TPP1; CLN2; GIG1; UNQ267; PRO304 |
| 背景资料 | Tripeptidyl-peptidase 1, also known as Lysosomal pepstatin-insensitive protease, is an enzyme that in humans is encoded by the TPP1 gene. The human gene TPP1 encodes a member of the sedolisin family of serine proteases. The human gene has 13 exons and locates at the chromosome band 11p15. This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2042-CAP | 96-well plate precoated with anti- human TPP1 antibody | 1 |
| EK2042-ST | Lyophilized recombinant human TPP1 standard | 10ng/tube×2 |
| EK2042-DA | Biotinylated anti- human TPP1 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 272 | 1160 | 3946 | 303 | 1187 | 4281 |
| Standard deviation | 10.88 | 54.52 | 205.19 | 15.45 | 70.03 | 303.95 |
| CV(%) | 4.0% | 4.7% | 5.2% | 5.1% | 5.9% | 7.1% |
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.037 | 0.106 | 0.181 | 0.310 | 0.558 | 0.943 | 1.552 | 2.170 |
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