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| 货号 | EK1753 |
|---|---|
| 产品名称 | Human TNFRSF12A/TWEAKR PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human TNFRSF12A/TWEAKR in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | E.coli, E28-P80 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | TNFRSF12A |
| 基因全名 | TNF receptor superfamily member 12A |
| Uniprot ID | Q9NP84 |
| 指标别名 | Tumor necrosis factor receptor superfamily member 12A; Fibroblast growth factor-inducible immediate-early response protein 14; FGF-inducible 14; Tweak-receptor; TweakR; CD266; TNFRSF12A; FN14 |
| 背景资料 | The gene for TNFRSF12A was initially recognized as a fibroblast growth factor inducible immediate early response gene Fn14 in mouse NIH 3T3 fibroblasts. This gene is mapped to 16p13.3. Human TNFRSF12A cDNA encodes a 129 amino acid residue type I transmembrane protein with a 27 aa signal peptide, a 53 aa extracellular domain, a 21 aa transmembrane domain and a 28 aa cytoplasmic domain. Human and mouse TNFRSF12A hold 82% aa sequence identity. TNFRSF12 is the tiniest member of the TNF receptor superfamily and has only one cysteine rich region in its extracellular domain. The TNFRSF12A cytoplasmic domain holds one TRAF binding motif which binds TRAFs 1, 2, and 3. TNFRSF12A binds its ligand TWEAK/TNFSF12A with high affinity to initiate a signal transduction cascade which subject to the cell type, causes different cellular responses such as cell death, cell proliferation, and angiogenesis. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1753-CAP | 96-well plate precoated with anti- human TNFRSF12A/TWEAKR antibody | 1 |
| EK1753-ST | Lyophilized recombinant human TNFRSF12A/TWEAKR standard | 10ng/tube×2 |
| EK1753-DA | Biotinylated anti- human TNFRSF12A/TWEAKR antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 47 | 209 | 855 | 58 | 247 | 903 |
| Standard deviation | 2.21 | 11.5 | 50.45 | 3.71 | 16.55 | 67.8 |
| CV(%) | 4.7% | 5.5% | 5.9% | 6.4% | 6.7% | 7.5% |
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.024 | 0.195 | 0.326 | 0.544 | 1.077 | 1.689 | 2.336 | 2.436 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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