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| 货号 | EK2038 |
|---|---|
| 产品名称 | Human TCN2 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human TCN2 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. |
| 免疫原 | NSO, E19-W427 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 0.78ng/ml-50ng/ml |
| 基因名称 | TCN2 |
| 基因全名 | transcobalamin 2 |
| Uniprot ID | P20062 |
| 指标别名 | Transcobalamin-2; TC-2; Transcobalamin II; TC II; TCII; TCN2; TC2 |
| 背景资料 | TCN2 (Transcobalamin II; also TC-2 or TC) is a 42-44 kDa, monomeric, secreted member of the eukaryotic cobalamin transport family of molecules. This gene is mapped to 22q12.2. This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2038-CAP | 96-well plate precoated with anti- human TCN2 antibody | 1 |
| EK2038-ST | Lyophilized recombinant human TCN2 standard | 50ng/tube×2 |
| EK2038-DA | Biotinylated anti- human TCN2 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 1065 | 5815 | 22584 | 1258 | 6014 | 2419 |
| Standard deviation | 55.38 | 308.20 | 1219.54 | 76.74 | 354.83 | 164.49 |
| CV(%) | 5.2% | 5.3% | 5.4% | 6.1% | 5.9% | 6.8% |
| Concentration(pg/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.015 | 0.129 | 0.223 | 0.423 | 0.689 | 1.097 | 1.676 | 2.111 |
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