货号 | EK1848 |
---|---|
产品名称 | Human SPOCK1/Testican-1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human SPOCK1/Testican-1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, R22-W439 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | SPOCK1 |
基因全名 | SPARC (osteonectin), cwcv and kazal like domains proteoglycan 1 |
Uniprot ID | Q08629 |
指标别名 | Testican-1; Protein SPOCK; SPOCK1; SPOCK; TIC1; TICN1 |
背景资料 | Testican-1 is a protein that in humans is encoded by the SPOCK1 gene. It is mapped to 5q31.2. This gene encodes the protein core of a seminal plasma proteoglycan containing chondroitin- and heparan-sulfate chains. The protein's function is unknown, although similarity to thyropin-type cysteine protease-inhibitors suggests its function may be related to protease inhibition. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1848-CAP | 96-well plate precoated with anti- human SPOCK1/Testican-1 antibody | 1 |
EK1848-ST | Lyophilized recombinant human SPOCK1/Testican-1 standard | 10ng/tube×2 |
EK1848-DA | Biotinylated anti- human SPOCK1/Testican-1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 21 | 67 | 435 | 26 | 73 | 474 |
Standard deviation | 0.95 | 2.88 | 22.19 | 1.72 | 5.04 | 34.60 |
CV(%) | 4.5% | 4.3% | 5.1% | 6.6% | 6.9% | 7.3% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.027 | 0.141 | 0.272 | 0.477 | 0.899 | 1.591 | 2.073 | 2.299 |
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