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| 货号 | EK2043 |
|---|---|
| 产品名称 | Human SPINK5 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human SPINK5 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | NSO, K23-E1064 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <50pg/ml |
| 检测范围 | 312pg/ml-20000pg/ml |
| 基因名称 | SPINK5 |
| 基因全名 | serine peptidase inhibitor, Kazal type 5 |
| Uniprot ID | Q9NQ38 |
| 指标别名 | Serine protease inhibitor Kazal-type 5; Lympho-epithelial Kazal-type-related inhibitor; LEKTI; SPINK5 |
| 背景资料 | Lympho-epithelial Kazal-type-related inhibitor (LEKTI) also known as serine protease inhibitor Kazal-type 5 (SPINK5) is a protein that in humans is encoded by the SPINK5 gene. This gene is mapped to 5q32. This gene encodes a multidomain serine protease inhibitor that contains 15 potential inhibitory domains. The encoded preproprotein is proteolytically processed to generate multiple protein products, which may exhibit unique activities and specificities. These proteins may play a role in skin and hair morphogenesis, as well as anti-inflammatory and antimicrobial protection of mucous epithelia. Mutations in this gene may result in Netherton syndrome, a disorder characterized by ichthyosis, defective cornification, and atopy. This gene is present in a gene cluster on chromosome 5. Alternative splicing results in multiple transcript variants. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2043-CAP | 96-well plate precoated with anti- human SPINK5 antibody | 1 |
| EK2043-ST | Lyophilized recombinant human SPINK5 standard | 20ng/tube×2 |
| EK2043-DA | Biotinylated anti- human SPINK5 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 436 | 1531 | 7423 | 537 | 2009 | 8417 |
| Standard deviation | 20.93 | 87.27 | 341.46 | 27.39 | 118.53 | 538.69 |
| CV(%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
| Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.047 | 0.112 | 0.151 | 0.276 | 0.509 | 0.905 | 1.549 | 2.294 |
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