货号 | EK2025 |
---|---|
产品名称 | Human SMPD1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human SMPD1 in cell culture supernates, serum and plasma (heparin). |
免疫原 | CHO, L47-C631 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | SMPD1 |
基因全名 | sphingomyelin phosphodiesterase 1 |
Uniprot ID | P17405 |
指标别名 | Sphingomyelin phosphodiesterase; Acid sphingomyelinase; aSMase; SMPD1; ASM |
背景资料 | Sphingomyelin phosphodiesterase 1 (SMPD1), also known as acid sphingomyelinase (ASM), is an enzyme that in humans is encoded by the SMPD1 gene. This gene is mapped to 11p15.4. The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. Sphingomyelin phosphodiesterase 1 belongs to the sphingomyelin phosphodiesterase family. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2025-CAP | 96-well plate precoated with anti- human SMPD1 antibody | 1 |
EK2025-ST | Lyophilized recombinant human SMPD1 standard | 10ng/tube×2 |
EK2025-DA | Biotinylated anti- human SMPD1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 33 | 119 | 417 | 35 | 121 | 452 |
Standard deviation | 1.42 | 6.19 | 19.18 | 2.14 | 7.14 | 33.45 |
CV(%) | 4.3% | 5.2% | 4.6% | 6.1% | 5.9% | 7.4% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.074 | 0.140 | 0.230 | 0.369 | 0.636 | 1.058 | 1.702 | 2.288 |
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