货号 | EK1862 |
---|---|
产品名称 | Human SEZ6L PicoKine ELISA Kit |
产品描述 | For quantitative detection of human SEZ6L in cell culture supernates, serum and plasma (heparin). |
免疫原 | NSO, L29-I1024 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.1ng/ml |
检测范围 | 0.312ng/ml-20ng/ml |
基因名称 | SEZ6L |
基因全名 | seizure related 6 homolog like |
Uniprot ID | Q9BYH1 |
指标别名 | Seizure 6-like protein; SEZ6L; KIAA0927; UNQ2542/PRO6094 |
背景资料 | Seizure 6-like protein is a protein that in humans is encoded by the SEZ6L gene. This gene is mapped to 22q12.1. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1862-CAP | 96-well plate precoated with anti- human SEZ6L antibody | 1 |
EK1862-ST | Lyophilized recombinant human SEZ6L standard | 20ng/tube×2 |
EK1862-DA | Biotinylated anti- human SEZ6L antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 489 | 1734 | 10155 | 544 | 2012 | 11046 |
Standard deviation | 31.30 | 97.10 | 710.85 | 23.39 | 114.68 | 839.50 |
CV(%) | 6.4% | 5.6% | 7.0% | 4.3% | 5.7% | 7.6% |
Concentration(ng/ml) | 0 | 0.3 | 0.63 | 1.25 | 2.5 | 5 | 10 | 20 |
O.D. | 0.015 | 0.088 | 0.152 | 0.266 | 0.486 | 0.828 | 1.446 | 2.065 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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