货号 | EK1712 |
---|---|
产品名称 | Human SerpinF2/Alpha-2-antiplasmin PicoKine ELISA Kit |
产品描述 | For quantitative detection of human SerpinF2 in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO,N40-K491 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 1.56ng/ml-100ng/ml |
基因名称 | SERPINF2 |
基因全名 | serpin family F member 2 |
Uniprot ID | Q9QY10 |
指标别名 | Fibroblast growth factor-binding protein 1; FGF-BP; FGF-BP1; FGF-binding protein 1; FGFBP-1; Growth factor-binding protein 1; Fgfbp1 |
背景资料 | Human SerpinF2 encodes a member of the serpin family of serine protease inhibitors. The protein is a major inhibitor of plasmin, which degrades fibrin and various other proteins. Consequently, the proper function of this gene has a major role in regulating the blood clotting pathway. Mutations in this gene result in alpha-2-plasmin inhibitor deficiency, which is characterized by severe hemorrhagic diathesis. Multiple transcript variants encoding different isoforms have been found for this gene. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1712-CAP | 96-well plate precoated with anti- human SerpinF2 antibody | 1 |
EK1712-ST | Lyophilized recombinant human SerpinF2 standard | 100ng/tubex2 |
EK1712-DA | Biotinylated anti- human SerpinF2 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 1883 | 10402 | 40754 | 1930 | 12110 | 43978 |
Standard deviation | 114.86 | 488.89 | 2282.2 | 123.52 | 884.03 | 3430.28 |
CV(%) | 6.1% | 4.7% | 5.6% | 6.4% | 7.3% | 7.8% |
Concentration(ng/ml) | 0 | 1.56 | 3.13 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.074 | 0.253 | 0.335 | 0.536 | 0.815 | 1.219 | 1.671 | 1.915 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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