货号 | EK1874 |
---|---|
产品名称 | Human Serpin A12 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Serpin A12 in cell culture supernates, serum and plasma (heparin, EDTA) and urine. |
免疫原 | NSO, L21-K414 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | SERPINA12 |
基因全名 | serpin family A member 12 |
Uniprot ID | Q8IW75 |
指标别名 | Serpin A12; OL-64; Visceral adipose tissue-derived serine protease inhibitor; Vaspin; Visceral adipose-specific serpin; SERPINA12 |
背景资料 | This gene is mapped to 14q32.13. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1874-CAP | 96-well plate precoated with anti- human Serpin A12 antibody | 1 |
EK1874-ST | Lyophilized recombinant human Serpin A12 standard | 10ng/tube×2 |
EK1874-DA | Biotinylated anti- human Serpin A12 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 212 | 1109 | 5465 | 232 | 1235 | 6154 |
Standard deviation | 11.24 | 68.76 | 245.93 | 10.90 | 79.04 | 332.32 |
CV(%) | 5.3% | 6.2% | 4.5% | 4.7% | 6.4% | 5.4% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.025 | 0.160 | 0.263 | 0.491 | 0.852 | 1.290 | 1.878 | 2.305 |
典型操作程序:
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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