货号 | EK1634 |
---|---|
产品名称 | Human Serpin A1/AAT PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Serpin A1 in cell culture supernates, serum, plasma (heparin, EDTA) and urine. |
免疫原 | NSO,E25-K418 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 1.56ng/ml-100ng/ml |
基因名称 | Serpin A1 |
基因全名 | serpin family A member 1 |
Uniprot ID | P01009 |
指标别名 | Alpha-1-antitrypsin; Alpha-1 protease inhibitor; Alpha-1-antiproteinase; Serpin A1; Short peptide from AAT; SPAAT; SERPINA1; AAT; PI; PRO0684; PRO2209 |
背景资料 | Alpha-1-antitrypsin or α1-antitrypsin (A1AT, A1A, or AAT) is a protein belonging to the serpin superfamily. It is encoded in humans by the SERPINA1 gene. The gene is located on the long arm of the fourteenth chromosome (14q32.1).The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver(infantile hepatic cirrhosis). |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1634-CAP | 96-well plate precoated with anti- Human Serpin A1 antibody | 1 |
EK1634-ST | Lyophilized recombinant Human Serpin A1 standard | 100ng/tubex2 |
EK1634-DA | Biotinylated anti- Human Serpin A1 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 2451 | 15117 | 38332 | 2323 | 15142 | 40979 |
Standard deviation | 144.6 | 1179.12 | 2529.91 | 157.96 | 1423.34 | 3114.4 |
CV(%) | 5.9% | 7.8% | 6.6% | 6.8% | 9.4% | 7.6% |
Concentration(ng/ml) | 0 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 |
O.D. | 0.083 | 0.112 | 0.237 | 0.403 | 0.679 | 1.050 | 1.622 | 2.128 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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