货号 | EK1755 |
---|---|
产品名称 | Human ROBO1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human ROBO1 in cell culture supernates, serum and plasma (heparin). |
免疫原 | NSO, Q26-P897 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | ROBO1 |
基因全名 | roundabout guidance receptor 1 |
Uniprot ID | Q9Y6N7 |
指标别名 | Roundabout homolog 1; Deleted in U twenty twenty; H-Robo-1; ROBO1; DUTT1 |
背景资料 | Roundabout homolog 1 is a protein that in humans is encoded by the ROBO1 gene. This gene is mapped to 3p12.3. The product of this gene is a member of the immunoglobulin gene superfamily and encodes an integral membrane protein that functions in axon guidance and neuronal precursor cell migration. This receptor is activated by SLIT-family proteins, resulting in a repulsive effect on glioma cell guidance in the developing brain. A related gene is located at an adjacent region on chromosome 3. Multiple transcript variants encoding different isoforms have been found for this gene. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1755-CAP | 96-well plate precoated with anti- human ROBO1 antibody | 1 |
EK1755-ST | Lyophilized recombinant human ROBO1 standard | 10ng/tube×2 |
EK1755-DA | Biotinylated anti- human ROBO1 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 112 | 381 | 1617 | 121 | 423 | 1768 |
Standard deviation | 5.71 | 20.4 | 69.53 | 5.93 | 28.3 | 139.7 |
CV(%) | 5.1% | 5.4% | 4.3% | 4.9% | 6.7% | 7.9% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.019 | 0.117 | 0.154 | 0.342 | 0.632 | 1.049 | 2.008 | 2.386 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知,且每位咨询者购买情况、提问时间等不同,为此以下回复仅对提问者3天内有效,其他网友仅供参考!若由此给您带来不便请多多谅解,谢谢!
服务热线
0771-3293894