货号 | EK1834 |
---|---|
产品名称 | Human REG3A PicoKine ELISA Kit |
产品描述 | For quantitative detection of human REG3A in cell culture supernates, serum and plasma (heparin). |
免疫原 | NSO, E27-D175 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <0.1ng/ml |
检测范围 | 0.78ng/ml-50ng/ml |
基因名称 | REG3A |
基因全名 | regenerating family member 3 alpha |
Uniprot ID | Q06141 |
指标别名 | Regenerating islet-derived protein 3-alpha; REG-3-alpha; Hepatointestinal pancreatic protein; HIP; PAP; Human proislet peptide; Pancreatitis-associated protein 1; Regenerating islet-derived protein III-alpha; Reg III-alpha; REG3A; PAP1 |
背景资料 | Regenerating islet-derived protein 3 alpha (or Regenerating islet-derived protein III-alpha) formerly known as HIP/PAP (Hepatocarcinoma-Intestine-Pancreas/Pancreatitis-Associated Protein) and peptide 23 is a protein that in humans is encoded by the REG3A gene. This gene is mapped to 2p12. It encodes a pancreatic secretory protein that may be involved in cell proliferation or differentiation. It has similarity to the C-type lectin superfamily. The enhanced expression of this gene is observed during pancreatic inflammation and liver carcinogenesis. The mature protein also functions as an antimicrobial protein with antibacterial activity. Alternate splicing results in multiple transcript variants that encode the same protein. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1834-CAP | 96-well plate precoated with anti- human REG3A antibody | 1 |
EK1834-ST | Lyophilized recombinant human REG3A standard | 50ng/tube×2 |
EK1834-DA | Biotinylated anti- human REG3A antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | ||||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 | |
n | 16 | 16 | 16 | 24 | 24 | 24 | |
Mean(pg/ml) | 1176 | 6316 | 19450 | 1433 | 6401 | 19872 | |
Standard deviation | 58.80 | 385.28 | 836.35 | 65.92 | 409.66 | 1430.78 | |
CV(%) | 5.0% | 6.1% | 4.3% | 4.6% | 6.4% | 7.2% |
Concentration(ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
O.D. | 0.004 | 0.010 | 0.033 | 0.104 | 0.280 | 1.805 | 1.429 | 2.499 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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