货号 | EK1812 |
---|---|
产品名称 | Human REG1A PicoKine ELISA Kit |
产品描述 | For quantitative detection of human REG1A in cell culture supernates, serum and plasma (heparin). |
免疫原 | NSO, Q23-N166 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | REG1A |
基因全名 | regenerating family member 1 alpha |
Uniprot ID | P05451 |
指标别名 | Lithostathine-1-alpha; Islet cells regeneration factor; ICRF; Islet of Langerhans regenerating protein; REG; Pancreatic stone protein; PSP; Pancreatic thread protein; PTP; Regenerating islet-derived protein 1-alpha; REG-1-alpha; Regenerating protein I alpha; REG1A; PSPS1 |
背景资料 | This gene is a type I subclass member of the Reg gene family. It is mapped to 2p12. The Reg gene family is a multigene family grouped into four subclasses, types I, II, III and IV, based on the primary structures of the encoded proteins. This gene encodes a protein that is secreted by the exocrine pancreas. It is associated with islet cell regeneration and diabetogenesis and may be involved in pancreatic lithogenesis. Reg family members REG1B, REGL, PAP and this gene are tandemly clustered on chromosome 2p12 and may have arisen from the same ancestral gene by gene duplication. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1812-CAP | 96-well plate precoated with anti- human REG1A antibody | 1 |
EK1812-ST | Lyophilized recombinant human REG1A standard | 10ng/tube×2 |
EK1812-DA | Biotinylated anti- human REG1A antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 89 | 786 | 1169 | 121 | 845 | 1307 |
Standard deviation | 4.63 | 51.09 | 66.63 | 8.35 | 49.01 | 82.34 |
CV(%) | 5.2% | 6.5% | 5.7% | 6.9% | 5.8% | 6.3% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 | |
O.D. | 0.136 | 0.151 | 0.193 | 0.281 | 0.409 | 0.691 | 1.344 | 2.338 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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