货号 | EK2104 |
---|---|
产品名称 | Human QPCT PicoKine ELISA Kit |
产品描述 | For quantitative detection of human QPCT in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, V29-L361 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | QPCT |
基因全名 | glutaminyl-peptide cyclotransferase |
Uniprot ID | Q16769 |
指标别名 | Glutaminyl-peptide cyclotransferase; Glutaminyl cyclase; QC; Sqc; Glutaminyl-tRNA cyclotransferase; Glutamyl cyclase; EC; QPCT |
背景资料 | Glutaminyl-peptide cyclotransferase is an enzyme that in humans is encoded by the QPCT gene. It is mapped to 2p22.2. This gene encodes human pituitary glutaminyl cyclase, which is responsible for the presence of pyroglutamyl residues in many neuroendocrine peptides. The amino acid sequence of this enzyme is 86% identical to that of bovine glutaminyl cyclase. |
显色时间 | 25-30 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2104-CAP | 96-well plate precoated with anti- human QPCT antibody | 1 |
EK2104-ST | Lyophilized recombinant human QPCT standard | 10ng/tube×2 |
EK2104-DA | Biotinylated anti- human QPCT antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 265 | 1264 | 4789 | 253 | 1258 | 4691 |
Standard deviation | 15.37 | 58.14 | 325.65 | 16.69 | 61.64 | 318.98 |
CV(%) | 5.8% | 4.6% | 6.8% | 6.6% | 4.9% | 6.8% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.021 | 0.079 | 0.142 | 0.266 | 0.483 | 0.838 | 1.510 | 2.124 |
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