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| 货号 | EK2087 |
|---|---|
| 产品名称 | Human PTH1R PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human PTH1R in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
| 免疫原 | CHO, Y23-M189 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | PTH1R |
| 基因全名 | parathyroid hormone 1 receptor |
| Uniprot ID | Q03431 |
| 指标别名 | Parathyroid hormone/parathyroid hormone-related peptide receptor; PTH/PTHrP type I receptor; PTH/PTHr receptor; Parathyroid hormone 1 receptor; PTH1 receptor; PTH1R; PTHR; PTHR1 |
| 背景资料 | Parathyroid hormone/parathyroid hormone-related peptide receptor, also known as parathyroid hormone 1 receptor (PTH1R), is a protein that in humans is encoded by the PTH1R gene. It is mapped to 3p21.31. The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK2087-CAP | 96-well plate precoated with anti- human PTH1R antibody | 1 |
| EK2087-ST | Lyophilized recombinant human PTH1R standard | 10ng/tube×2 |
| EK2087-DA | Biotinylated anti- human PTH1R antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 46 | 139 | 566 | 52 | 171 | 643 |
| Standard deviation | 2.21 | 7.23 | 30.56 | 2.65 | 9.41 | 40.51 |
| CV(%) | 4.8% | 5.2% | 5.4% | 5.1% | 5.5% | 6.3% |
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.008 | 0.092 | 0.159 | 0.308 | 0.626 | 1.104 | 1.872 | 2.474 |
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