货号 | EK1827 |
---|---|
产品名称 | Human Plexin B3 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Plexin B3 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, H45-Q1255 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 93.6pg/ml-6000pg/ml |
基因名称 | PLXNB3 |
基因全名 | plexin B3 |
Uniprot ID | Q9ULL4 |
指标别名 | Plexin-B3; PLXNB3; KIAA1206; PLXN6 |
背景资料 | Plexin-B3 is a protein that in humans is encoded by the PLXNB3 gene. Ths gene is mapped to Xq28. The protein encoded by this gene is a member of the plexin family. It functions as a receptor for semaphorin 5A, and plays a role in axon guidance, invasive growth and cell migration. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1827-CAP | 96-well plate precoated with anti- human Plexin B3 antibody | 1 |
EK1827-ST | Lyophilized recombinant human Plexin B3 standard | 10ng/tube×2 |
EK1827-DA | Biotinylated anti- human Plexin B3 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 156 | 984 | 3201 | 169 | 992 | 3321 |
Standard deviation | 9.52 | 53.14 | 208.07 | 7.94 | 69.44 | 259.04 |
CV(%) | 6.1% | 5.4% | 6.5% | 4.7% | 7.0% | 7.8% |
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.021 | 0.110 | 0.177 | 0.334 | 0.587 | 0.994 | 1.635 | 2.389 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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