货号 | EK1944 |
---|---|
产品名称 | Human PLA2G2A PicoKine ELISA Kit |
产品描述 | For quantitative detection of human PLA2G2A in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | sf21, N21-C144 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | PLA2G2A |
基因全名 | phospholipase A2 group IIA |
Uniprot ID | P14555 |
指标别名 | Phospholipase A2, membrane associated; GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2; NPS-PLA2; Phosphatidylcholine 2-acylhydrolase 2A; PLA2G2A; PLA2B; PLA2L; RASF-A |
背景资料 | Phospholipase A2, membrane associated is an enzyme that in humans is encoded by the PLA2G2A gene. It is mapped to 1p36.13. The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5'UTRs have been found for this gene. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1944-CAP | 96-well plate precoated with anti- human PLA2G2A antibody | 1 |
EK1944-ST | Lyophilized recombinant human PLA2G2A standard | 10ng/tube×2 |
EK1944-DA | Biotinylated anti- human PLA2G2A antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 249 | 1511 | 3890 | 265 | 1585 | 4121 |
Standard deviation | 13.20 | 93.68 | 171.16 | 18.82 | 76.08 | 259.62 |
CV(%) | 5.3% | 6.2% | 4.4% | 7.1% | 4.8% | 6.3% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.027 | 0.111 | 0.195 | 0.346 | 0.614 | 1.040 | 1.697 | 2.255 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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