货号 | EK1700 |
---|---|
产品名称 | Human PDGF-DD PicoKine ELISA Kit |
产品描述 | For quantitative detection of human PDGF-DD in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,S250-R370 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | PDGFD |
基因全名 | platelet derived growth factor D |
Uniprot ID | Q9GZP0 |
指标别名 | Platelet-derived growth factor D; PDGF-D;Iris-expressed growth factor; Spinal cord-derived growth factor B; SCDGF-B; PDGFD latent form; PDGFD receptor-binding form; PDGFD; IEGF; SCDGFB; MSTP036; UNQ1899/PRO4345 |
背景资料 | Platelet-derived growth factor (PDGF) is one of numerous growth factors that regulate cell growth and division.The PDGF family consists of PDGF-A, -B, -C and -D, which form either homo- or heterodimers (PDGF-AA, -AB, -BB, -CC, -DD). The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a core motif of eight cysteines, seven of which are found in this factor. This gene product only forms homodimers and, therefore, does not dimerize with the other three family members. It differs from alpha and beta members of this family in having an unusual N-terminal domain, the CUB domain. Two splice variants have been identified for this gene. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1700-CAP | 96-well plate precoated with anti- human PDGF-DD antibody | 1 |
EK1700-ST | Lyophilized recombinant human PDGF-DD standard | 10ng/tubex2 |
EK1700-DA | Biotinylated anti- human PDGF-DD antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 51 | 222 | 762 | 57 | 229 | 841 |
Standard deviation | 2.81 | 9.1 | 43.4 | 3.53 | 14.89 | 61.39 |
CV(%) | 5.5% | 4.1% | 5.7% | 6.2% | 6.5% | 7.3% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.042 | 0.122 | 0.175 | 0.276 | 0.520 | 0.952 | 1.408 | 2.370 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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