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| 货号 | EK0956 |
|---|---|
| 产品名称 | Human PDGF-BB PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human PDGF-BB in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | E.coli, S82-T190 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | PDGFB |
| 基因全名 | platelet-derived growth factor beta polypeptide |
| Uniprot ID | P01127 |
| 指标别名 | Becaplermin| c sis| FLJ12858| Oncogene SIS| PDG B chain| PDGB| PDGF 2| PDGF B| PDGF B chain| PDGF subunit B| PDGF-2| PDGF2| Pdgfb| PDGFB/COL1A1 fusion gene| PDGFB_HUMAN| Platelet derived growth factor 2| Platelet derived growth factor B chain| Platelet derived growth factor beta polypeptide| Platelet derived growth factor beta polypeptide (oncogene SIS)| Platelet derived growth factor subunit B| Platelet-derived growth factor B chain| Platelet-derived growth factor beta polypeptide| Platelet-derived growth factor subunit B| Proto oncogene c Sis| Proto-oncogene c-Sis| Simian sarcoma viral (v sis) oncogene homolog| SIS| SSV| V sis platelet derived growth factor beta polypeptide |
| 背景资料 | Platelet-derived growth factor subunit B is a protein that in humans is encoded by the PDGFB gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. This gene product can exist either as a homodimer(PDGF-BB) or as a heterodimer with the platelet-derived growth factor alpha polypeptide(PDGF-AB), where the dimers are connected by disulfide bonds. This gene is mapped to 22q13.1. Growth factor plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. This gene plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. |
| 显色时间 | 20-25 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0956-CAP | 96-well plate precoated with anti- human PDGF-BB antibody | 1 |
| EK0956-ST | Lyophilized recombinant human PDGF-BB standard | 10ng/tube×2 |
| EK0956-DA | Biotinylated anti- human PDGF-BB antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 94 | 199 | 788 | 88 | 194 | 725 |
| Standard deviation | 4.13 | 12.53 | 39.4 | 5.19 | 12.8 | 36.25 |
| CV(%) | 4.4% | 6.3% | 5% | 5.9% | 6.6% | 5% |
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.046 | 0.074 | 0.119 | 0.198 | 0.439 | 0.954 | 1.971 | 2.527 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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