货号 | EK1890 |
---|---|
产品名称 | Human P4HB PicoKine ELISA Kit |
产品描述 | For quantitative detection of human P4HB in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, D18-L508 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 93.8pg/ml-6000pg/ml |
基因名称 | P4HB |
基因全名 | prolyl 4-hydroxylase subunit beta |
Uniprot ID | P07237 |
指标别名 | Protein disulfide-isomerase; PDI; Cellular thyroid hormone-binding protein; Prolyl 4-hydroxylase subunit beta; p55; P4HB; ERBA2L; PDI; PDIA1; PO4DB |
背景资料 | P4HB is a multifunctional and highly abundant enzyme that is part of the protein disulfide isomerase family. It is mapped to 17q25.3. When present as a tetramer consisting of two alpha and two beta subunits, P4HB has a role in hydroxylation of prolyl residues in preprocollagen. P4HB is a disulfide isomerase containing two thioredoxin domains that catalyze the formation, breakage and rearrangement of disulfide bonds. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1890-CAP | 96-well plate precoated with anti- human P4HB antibody | 1 |
EK1890-ST | Lyophilized recombinant human P4HB standard | 10ng/tube×2 |
EK1890-DA | Biotinylated anti- human P4HB antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 155 | 785 | 2454 | 177 | 790 | 2763 |
Standard deviation | 8.84 | 55.74 | 107.98 | 10.09 | 48.19 | 212.75 |
CV(%) | 5.7% | 7.1% | 4.4% | 5.7% | 6.1% | 7.7% |
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.020 | 0.081 | 0.154 | 0.259 | 0.522 | 0.837 | 1.495 | 2.314 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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