货号 | EK1946 |
---|---|
产品名称 | Human Neudesin PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Neudesin in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, G32-F172 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 93.6pg/ml-6000pg/ml |
基因名称 | NENF |
基因全名 | neudesin neurotrophic factor |
Uniprot ID | Q9UMX5 |
指标别名 | Neudesin; Cell immortalization-related protein 2; Neuron-derived neurotrophic factor; Protein GIG47; Secreted protein of unknown function; SPUF protein; NENF; CIR2; SPUF |
背景资料 | This gene encodes a neurotrophic factor that may play a role in neuron differentiation and development. A pseudogene of this gene is found on chromosome 12. Alternate splicing of this gene results in multiple transcript variants. It is mapped to 1q32.3. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1946-CAP | 96-well plate precoated with anti- human Neudesin antibody | 1 |
EK1946-ST | Lyophilized recombinant human Neudesin standard | 10ng/tube×2 |
EK1946-DA | Biotinylated anti- human Neudesin antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 124 | 715 | 2078 | 159 | 739 | 2238 |
Standard deviation | 7.69 | 32.18 | 118.45 | 7.79 | 42.86 | 140.99 |
CV(%) | 6.2% | 4.5% | 5.7% | 4.9% | 5.8% | 6.3% |
Concentration(pg/ml) | 0 | 93.6 | 187 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.058 | 0.119 | 0.181 | 0.257 | 0.482 | 0.910 | 1.522 | 2.263 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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