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| 货号 | EK1961 |
|---|---|
| 产品名称 | Human Nectin-3/CD113 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human Nectin-3/CD113 in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, G58-T404 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <20pg/ml |
| 检测范围 | 125pg/ml-8000pg/ml |
| 基因名称 | NECTIN3 |
| 基因全名 | nectin cell adhesion molecule 3 |
| Uniprot ID | Q9NQS3 |
| 指标别名 | Nectin-3; CDw113; Nectin cell adhesion molecule 3; Poliovirus receptor-related protein 3; CD113; NECTIN3; PRR3; PVRL3 |
| 背景资料 | Poliovirus receptor-related 3 (PVRL3), also known as nectin-3 and CD113, is a human protein of the immunoglobulin superfamily which forms part of adherens junctions. It is mapped to 19q13.32. This gene encodes a member of the nectin family of proteins, which function as adhesion molecules at adherens junctions. This family member interacts with other nectin-like proteins and with afadin, a filamentous actin-binding protein involved in the regulation of directional motility, cell proliferation and survival. This gene plays a role in ocular development involving the ciliary body. Mutations in this gene are believed to result in congenital ocular defects. Alternative splicing results in multiple transcript variants. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1961-CAP | 96-well plate precoated with anti- human Nectin-3/CD113 antibody | 1 |
| EK1961-ST | Lyophilized recombinant human Nectin-3/CD113 standard | 10ng/tube×2 |
| EK1961-DA | Biotinylated anti- human Nectin-3/CD113 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 159 | 873 | 3809 | 239 | 939 | 3980 |
| Standard deviation | 10.65 | 45.40 | 217.11 | 15.06 | 50.71 | 163.18 |
| CV(%) | 6.7% | 5.2% | 5.7% | 6.3% | 5.4% | 4.1% |
| Concentration(pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.110 | 0.183 | 0.230 | 0.386 | 0.596 | 0.991 | 1.661 | 2.295 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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