0
The Bosterbio OneStep ELISA kit is a solid phase direct ELISA sandwich kit. Instead of adding samples detection antibody and ABC-HRP separately, The OneStep ELISA kit allows the user to add standards, samples and controls to wells in one step, along with the incubation buffer. After a simple washing step, an enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. The enzyme catalyzes the substrate yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. The intensity of color developed is directly proportional to the concentration of target protein in the samples. A standard curve is generated relating color intensity to the concentration of target protein.
| Product Name | Human Myoglobin OneStep ELISA Kit |
|---|---|
| SKU/Catalog Number | EK7069 |
| Description | Human Myoglobin OneStep ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips. |
| Cite This Product | Human Myoglobin OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7069) |
| Validated Species | Human |
| Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Cross Reactivity | There is no detectable cross-reactivity. |
| Pack Size | 96wells/kit, with removable strips. |
| Sensitivity | 25 ng/ml *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive. |
|---|---|
| Assay Range | 25-1000 ng/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
| Sample Type | Serum and Plasma *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
| Storage | Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Quantity |
| 1. Microwell coated with murine monoclonal anti-myoglobin. | 12x8x1 |
| 2. Reference Standard Set | 0.5 ml |
| 3. Sample Diluent | 25 ml |
| 4. Enzyme Conjugate Reagent | 22 ml |
| 5. TMB Reagent | 11 ml |
| 6. Stop Solution | 11 ml |
| 7. Wash Concentrate 20x: 1 Bottle | 25 ml |
1. Distilled or deionized water
2. Precision pipettes
3. Disposable pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. ELISA reader capable of reading absorbance at 450nm
5. Absorbance paper or paper towel
6. Graph paper
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | MB |
|---|---|
| Uniprot ID | P02144 |
*if product is indicated to react with multiple species, protein info is based on the human gene.
1. For Research Use Only. Not for use in diagnostic procedures.
2. Potential biohazardous materials: The calibrator and controls contain human source components, which have been tested and found non-reactive for hepatitis B surface antigen as well as HIV antibody with FDA licensed reagents. However, as there is no test method that can offer complete assurance that HIV, Hepatitis B virus or other infectious agents are absent, these reagents should be handled at the Biosafety Level 2, as recommended in the Centers for Disease Control/National Institutes of Health manual, "Biosafety in Microbiological and Biomedical Laboratories." 1984
3. Optimal results will be obtained by strict adherence to the test protocol. Precise pipetting as well as following the exact time and temperature requirements is essential.
4. Do not pipette by mouth. Do not smoke, eat, or drink in the areas in which specimens or kit reagents are handled.
5. The components in this kit are intended for use as an integral unit. The components of different lots should not be mixed.
6. This product contains components preserved with sodium azide. Sodium azide may react with lead and copper plumbing to form explosive metal azide. On disposal, flush with a large volume of water.
1. Collect blood specimens and separate the serum immediately.
2. Typically, specimens may be stored refrigerated at (2-8. C) for 5 days. If storage time exceeds 5 days, store frozen at (-20°C) for up to one month.
3. Avoid multiple freeze-thaw cycles.
4. Prior to assay, frozen sera should be completely thawed and mixed well.
5. Do not use grossly lipemic specimens.
Prepare 1X Wash buffer by adding the contents of the bottle (25 ml, 20X) to 475 ml of distilled or deionized water. Store at room temperature (20-25. C).
1. All reagents should be brought to room temperature (20-25°C) before use.
2. Patient serum and control serum should be diluted 10 fold before use. Prepare a series of small tubes (such as 1.5 ml microcentrifuge tubes) and mix 20ul serum with 180ul (0.18 ml) Sample Diluent. PLEASE DO NOT DILUTE THE STANDARDS--THEY HAVE ALREADY BEEN PRE-DILUTED 10-FOLD
3. Samples with expected myoglobin concentrations over 1000 ng/ml may be quantitated by further dilution 10-fold with sample diluent.
4. Prepare 1X Wash buffer by adding the contents of the bottle (25 ml, 20X) to 475 ml of distilled or deionized water. Store at room temperature (20-25°C).
1. Patient serum and control serum should be diluted 10 fold before use. Prepare a series of small tubes (such as 1.5 ml microcentrifuge tubes) and mix 20ul serum or plasma with 180 ul (0.18 ml) Sample Diluent. PLEASE DO NOT DILUTE THE STANDARDS -- THEY HAVE ALREADY BEEN PRE-DILUTED 10-FOLD
2. Secure the desired number of coated wells in the holder.
3. Dispense 20ul of myoglobin standards, diluted specimens and diluted controls into the appropriate wells.
4. Dispense 200ul of Enzyme Conjugate Reagent into each well.
5. Thoroughly mix for 30 seconds. It is very important to mix completely.
6. Incubate at room temperature (20-25°C) for 45 minutes.
7. Remove the incubation mixture by flicking plate contents into a waste container.
8. Remove liquid from all wells. Wash wells three times with 300ul of 1X wash buffer. Blot on absorbance paper or paper towel.
9. Strike the wells sharply onto absorbent paper or paper towels to remove all residual water drops.
10. Dispense 100ul of TMB Reagent solution into each well. Gently mix for 5 seconds.
11. Incubate at room temperature for 20 minutes.
12. Stop the reaction by adding 100ul of Stop Solution to each well.
13. Gently mix 30 seconds. It is important to make sure that all the blue color changes to yellow color completely.
14. Read absorbance at 450 nm with a microtiter well reader within 15 minutes.
温馨提示:因厂家更改产品包装、产地或者更换随机附件等没有任何提前通知，且每位咨询者购买情况、提问时间等不同，为此以下回复仅对提问者3天内有效，其他网友仅供参考！若由此给您带来不便请多多谅解，谢谢！
服务热线
0771-3293894
新浪微博
腾讯微博
QQ空间
客服
