货号 | EK2034 |
---|---|
产品名称 | Human Myeloblastin PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Myeloblastin in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | E.coli, I28-R248 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 62.5pg/ml-4000pg/ml |
基因名称 | PRTN3 |
基因全名 | proteinase 3 |
Uniprot ID | P24158 |
指标别名 | Myeloblastin; AGP7; C-ANCA antigen; Leukocyte proteinase 3; PR-3; PR3; Neutrophil proteinase 4; NP-4; P29; Wegener autoantigen; PRTN3; MBN |
背景资料 | Proteinase 3 also known as PRTN3 is an enzyme that in humans is encoded by the PRTN3 gene. This gene is mapped to 19p13.3. PRTN3 is a serine protease enzyme expressed mainly in neutrophil granulocytes. Its exact role in the function of the neutrophil is unknown, but, in human neutrophils, proteinase 3 contributes to the proteolytic generation of antimicrobial peptides. It is also the target of anti-neutrophil cytoplasmic antibodies (ANCAs) of the c-ANCA (cytoplasmic subtype) class, a type of antibody frequently found in the disease granulomatosis with polyangiitis. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2034-CAP | 96-well plate precoated with anti- human Myeloblastin antibody | 1 |
EK2034-ST | Lyophilized recombinant human Myeloblastin standard | 4ng/tube×2 |
EK2034-DA | Biotinylated anti- human Myeloblastin antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 109 | 326 | 1513 | 116 | 401 | 1765 |
Standard deviation | 4.58 | 14.02 | 62.03 | 7.66 | 27.27 | 125.32 |
CV(%) | 4.2% | 4.3% | 4.1% | 6.6% | 6.8% | 7.1% |
Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
O.D. | 0.032 | 0.068 | 0.082 | 0.113 | 0.221 | 0.478 | 1.271 | 2.472 |
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