货号 | EK1845 |
---|---|
产品名称 | Human MANF PicoKine ELISA Kit |
产品描述 | For quantitative detection of human MANF in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, L25-L182 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <15pg/ml |
检测范围 | 93.6pg/ml-6000pg/ml |
基因名称 | MANF |
基因全名 | mesencephalic astrocyte derived neurotrophic factor |
Uniprot ID | P55145 |
指标别名 | Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMET; MANF; ARMET; ARP |
背景资料 | MANF is a 20kDa protein which belongs to the ARMET family. This gene is mapped to 3p21.2. MANF was originally known as an arginine-rich region protein which was extremely mutated in a large number of tumors. MANF Expression is induced during ER stress, signifying that MANF takes part in protein quality control during ER stress. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1845-CAP | 96-well plate precoated with anti- human MANF antibody | 1 |
EK1845-ST | Lyophilized recombinant human MANF standard | 10ng/tube×2 |
EK1845-DA | Biotinylated anti- human MANF antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 156 | 733 | 2725 | 166 | 748 | 2871 |
Standard deviation | 9.67 | 47.65 | 125.35 | 8.47 | 55.35 | 195.23 |
CV(%) | 6.2% | 6.5% | 4.6% | 5.1% | 7.4% | 6.8% |
Concentration(pg/ml) | 0 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
O.D. | 0.108 | 0.161 | 0.188 | 0.313 | 0.606 | 1.226 | 1.697 | 2.337 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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