货号 | EK2061 |
---|---|
产品名称 | Human MAG PicoKine ELISA Kit |
产品描述 | For quantitative detection of human MAG in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, G20-P516 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | MAG |
基因全名 | myelin associated glycoprotein |
Uniprot ID | P20916 |
指标别名 | Myelin-associated glycoprotein; Siglec-4a; MAG; GMA |
背景资料 | Myelin-associated glycoprotein (MAG, Siglec-4) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NoGo-66 receptor(NgR). This gene is mapped to 19q13.12. The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2061-CAP | 96-well plate precoated with anti- human MAG antibody | 1 |
EK2061-ST | Lyophilized recombinant human MAG standard | 20ng/tube×2 |
EK2061-DA | Biotinylated anti- human MAG antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 384 | 2313 | 8445 | 492 | 2436 | 9458 |
Standard deviation | 17.28 | 131.84 | 388.47 | 25.09 | 143.72 | 699.89 |
CV(%) | 4.5% | 5.7% | 4.6% | 5.1% | 5.9% | 7.4% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.055 | 0.125 | 0.169 | 0.299 | 0.545 | 0.956 | 1.437 | 2.064 |
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