货号 | EK1687 |
---|---|
产品名称 | Human MADCAM-1 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human MADCAM-1 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,Q19-Q317 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 31.2pg/ml-2000pg/ml |
基因名称 | MADCAM1 |
基因全名 | mucosal vascular addressin cell adhesion molecule 1 |
Uniprot ID | Q13477 |
指标别名 | Mucosal addressin cell adhesion molecule 1; MAdCAM-1; hMAdCAM-1; MADCAM1 |
背景资料 | MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4/beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1687-CAP | 96-well plate precoated with anti- human MADCAM-1 antibody | 1 |
EK1687-ST | Lyophilized recombinant human MADCAM-1 standard | 10ng/tubex2 |
EK1687-DA | Biotinylated anti- human MADCAM-1 antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 72 | 241 | 1030 | 77 | 233 | 996 |
Standard deviation | 4.96 | 15.66 | 62.83 | 5.92 | 16.07 | 62.74 |
CV(%) | 6.9% | 6.5% | 6.1% | 7.7% | 6.9% | 6.3% |
Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
O.D. | 0.026 | 0.110 | 0.178 | 0.359 | 0.659 | 1.062 | 1.428 | 1.994 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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