货号 | EK1688 |
---|---|
产品名称 | Human Klotho PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Klotho in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,E34-S981 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | KL |
基因全名 | klotho |
Uniprot ID | Q9UEF7 |
指标别名 | Klotho; Klotho peptide; KL |
背景资料 | Klotho(KL) is a glycosylated protein that plays an important role in the regulation of phosphate and calcium homeostasis. This gene encodes a type-I membrane protein that is related to beta-glucosidases. Reduced production of this protein has been observed in patients with chronic renal failure (CRF), and this may be one of the factors underlying the degenerative processes (e.g., arteriosclerosis, osteoporosis, and skin atrophy) seen in CRF. Also, mutations within this protein have been associated with ageing and bone loss and alcohol consumption. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1688-CAP | 96-well plate precoated with anti- human Klotho antibody | 1 |
EK1688-ST | Lyophilized recombinant human Klotho standard | 10ng/tubex2 |
EK1688-DA | Biotinylated anti- human Klotho antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 308 | 1224 | 5567 | 337 | 1220 | 5449 |
Standard deviation | 15.4 | 79.56 | 239.38 | 20.55 | 102.48 | 321.49 |
CV(%) | 5% | 6.5% | 4.3% | 6.1% | 8.4% | 5.9% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.055 | 0.073 | 0.100 | 0.141 | 0.231 | 0.481 | 1.089 | 1.920 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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