货号 | EK1844 |
---|---|
产品名称 | Human JAM-C PicoKine ELISA Kit |
产品描述 | For quantitative detection of human JAM-C in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, V32-N241 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 46.9pg/ml-3000pg/ml |
基因名称 | JAM3 |
基因全名 | junctional adhesion molecule 3 |
Uniprot ID | Q9BX67 |
指标别名 | Junctional adhesion molecule C; JAM-C; JAM-2; Junctional adhesion molecule 3; JAM-3; Jam3; UNQ859/PRO1868 |
背景资料 | This gene is mapped to 11q25. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. The protein encoded by this immunoglobulin superfamily gene member is localized in the tight junctions between high endothelial cells. Unlike other proteins in this family, the this protein is unable to adhere to leukocyte cell lines and only forms weak homotypic interactions. The encoded protein is a member of the junctional adhesion molecule protein family and acts as a receptor for another member of this family. A mutation in an intron of this gene is associated with hemorrhagic destruction of the brain, subependymal calcification, and congenital cataracts. Alternative splicing results in multiple transcript variants. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1844-CAP | 96-well plate precoated with anti- human JAM-C antibody | 1 |
EK1844-ST | Lyophilized recombinant human JAM-C standard | 10ng/tube×2 |
EK1844-DA | Biotinylated anti- human JAM-C antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 90 | 422 | 1408 | 92 | 430 | 1466 |
Standard deviation | 4.86 | 23.63 | 57.73 | 4.32 | 27.09 | 104.09 |
CV(%) | 5.4% | 5.6% | 4.1% | 4.7% | 6.3% | 7.1% |
Concentration(pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.026 | 0.153 | 0.204 | 0.429 | 0.767 | 0.389 | 1.692 | 2.226 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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