货号 | EK1842 |
---|---|
产品名称 | Human JAM-B/JAM2 PicoKine ELISA Kit |
产品描述 | For quantitative detection of human JAM-B/JAM2 in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, F29-S238 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 15.6pg/ml-1000pg/ml |
基因名称 | JAM2 |
基因全名 | junctional adhesion molecule 2 |
Uniprot ID | P57087 |
指标别名 | Junctional adhesion molecule B; JAM-B; Junctional adhesion molecule 2; JAM-2; Vascular endothelial junction-associated molecule; VE-JAM; CD322; JAM2; C21orf43; VEJAM; UNQ219/PRO245 |
背景资料 | Junctional adhesion molecule B is a protein that in humans is encoded by the JAM2 gene. It is mapped to 21q21.3. This gene belongs to the immunoglobulin superfamily, and the junctional adhesion molecule (JAM) family. The protein encoded by this gene is a type I membrane protein that is localized at the tight junctions of both epithelial and endothelial cells. It acts as an adhesive ligand for interacting with a variety of immune cell types, and may play a role in lymphocyte homing to secondary lymphoid organs. Alternatively spliced transcript variants have been found for this gene. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1842-CAP | 96-well plate precoated with anti- human JAM-B/JAM2 antibody | 1 |
EK1842-ST | Lyophilized recombinant human JAM-B/JAM2 standard | 10ng/tube×2 |
EK1842-DA | Biotinylated anti- human JAM-B/JAM2 antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30 ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 27 | 190 | 408 | 31 | 213 | 467 |
Standard deviation | 1.13 | 12.73 | 22.44 | 2.11 | 12.57 | 28.49 |
CV(%) | 4.2% | 6.7% | 5.5% | 6.8% | 5.9% | 6.1% |
Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.012 | 0.147 | 0.264 | 0.463 | 0.819 | 1.613 | 2.165 | 2.793 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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