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The Bosterbio OneStep ELISA kit is a solid phase direct ELISA sandwich kit. Instead of adding samples detection antibody and ABC-HRP separately, The OneStep ELISA kit allows the user to add standards, samples and controls to wells in one step, along with the incubation buffer. After a simple washing step, an enzyme conjugate reagent is added into each well. After the excess enzyme conjugate is washed out, the substrate is added into each well. The enzyme catalyzes the substrate yielding a blue color (Amax = 370nm and 652nm) that changes to yellow (Amax = 450nm) upon addition of a sulfuric or phosphoric acid stop solution. The intensity of color developed is directly proportional to the concentration of target protein in the samples. A standard curve is generated relating color intensity to the concentration of target protein.
| Product Name | Human Intact Parathyroid Hormone (PTH) OneStep ELISA Kit |
|---|---|
| SKU/Catalog Number | EK7081 |
| Description | Human Intact Parathyroid Hormone (PTH) OneStep ELISA Kit, tested with Serum and Plasma. Format: 96wells/kit, with removable strips. |
| Cite This Product | Human Intact Parathyroid Hormone (PTH) OneStep ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7081) |
| Validated Species | Human |
| Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Cross Reactivity | There is no detectable cross-reactivity. |
| Pack Size | 96wells/kit, with removable strips. |
| Assay Range | Sample volume *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
|---|---|
| Sample Type | Serum and Plasma *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
| Storage | Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Quantity |
| 1. Microwells coated with Streptavidin | 12x8x1 |
| 2. PTH Standard 6: 1 Vial (lyophilized) | 0.75 ml |
| 3. PTH Controls: 2 Vials (lyophilized) | 0.75ml |
| 4. Anti-PTH-Biotin Reagent: 1 Bottle (Ready to use) | 7 ml |
| 5. Anti-PTH-HRP Enzyme Conjugate: 1 Bottle (Ready to use) | 7 ml |
| 6. Assay Diluent: 1 Bottle (ready to use) | 5 ml |
| 6. TMB Substrate: 1 Bottle (Ready to use) | 12 ml |
| 7. Stop Solution: 1 Bottle (Ready to use) | 12 ml |
| 8. 20X Wash Solution: 1 Bottle | 25 ml |
1. Distilled or deionized water
2. Precision pipettes
3. Disposable pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. ELISA reader capable of reading absorbance at 450nm
5. Absorbance paper or paper towel
6. Graph paper
You can check the tissue specificity below for information on selecting positive and negative control.
| Gene Name | PTH1R |
|---|---|
| Uniprot ID | Q03431 |
*if product is indicated to react with multiple species, protein info is based on the human gene.
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