货号 | EK1769 |
---|---|
产品名称 | Human IL-1RAcP/IL-1R3/IL1RAP PicoKine ELISA Kit |
产品描述 | For quantitative detection of human IL-1RAcP/IL-1R3/IL1RAP in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO, S21-T367 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <50pg/ml |
检测范围 | 312pg/ml-20000pg/ml |
基因名称 | IL1RAP |
基因全名 | interleukin 1 receptor accessory protein |
Uniprot ID | Q9NPH3 |
指标别名 | Interleukin-1 receptor accessory protein; IL-1 receptor accessory protein; IL-1RAcP; Interleukin-1 receptor 3; IL-1R-3; IL-1R3; IL1RAP; C3orf13, IL1R3 |
背景资料 | Interleukin-1 receptor accessory protein is a protein that in humans is encoded by the IL1RAP gene. It is mapped to 3q28. This gene encodes a component of the interleukin 1 receptor complex, which initiates signalling events that result in the activation of interleukin 1-responsive genes. Alternative splicing of this gene results in membrane-bound and soluble isoforms differing in their C-terminus. The ratio of soluble to membrane-bound forms increases during acute-phase induction or stress. |
显色时间 | 15-20 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1769-CAP | 96-well plate precoated with anti- human IL-1RAcP/IL-1R3/IL1RAP antibody | 1 |
EK1769-ST | Lyophilized recombinant human IL-1RAcP/IL-1R3/IL1RAP standard | 20ng/tube×2 |
EK1769-DA | Biotinylated anti- human IL-1RAcP/IL-1R3/IL1RAP antibody | 130μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 402 | 1364 | 5883 | 413 | 1472 | 6042 |
Standard deviation | 18.49 | 77.75 | 270.62 | 21.06 | 86.85 | 386.69 |
CV(%) | 4.6% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
Concentration(pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
O.D. | 0.016 | 0.035 | 0.046 | 0.086 | 0.186 | 0.500 | 1.146 | 2.111 |
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