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| 货号 | EK1707 |
|---|---|
| 产品名称 | Human HRG/HPRG PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human HRG in cell culture supernates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO,V19-K525 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | HRG |
| 基因全名 | histidine rich glycoprotein |
| Uniprot ID | Q6P1K1 |
| 指标别名 | Heme transporter HRG1; Heme-responsive gene 1 protein homolog; HRG-1; hHRG-1; Solute carrier family 48 member 1; SLC48A1; HRG1 |
| 背景资料 | HRG contains two cystatin-like domains and is located in plasma and platelets. The physiological function has not been determined but it is known that the protein binds heme, dyes and divalent metal ions. The encoded protein also has a peptide that displays antimicrobial activity against C. albicans, E. coli, S. aureus, P. aeruginosa, and E. faecalis. It can inhibit rosette formation and interacts with heparin, thrombospondin and plasminogen. Two of the protein's effects, the inhibition of fibrinolysis and the reduction of inhibition of coagulation, indicate a potential prothrombotic effect. Mutations in this gene lead to thrombophilia due to abnormal histidine-rich glycoprotein levels. |
| 显色时间 | 15-20 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1707-CAP | 96-well plate precoated with anti- human HRG antibody | 1 |
| EK1707-ST | Lyophilized recombinant human HRG standard | 10ng/tubex2 |
| EK1707-DA | Biotinylated anti- human HRG antibody | 130ul(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 244 | 1957 | 4517 | 246 | 1967 | 4596 |
| Standard deviation | 10.49 | 95.89 | 243.9 | 16.48 | 135.7 | 344.7 |
| CV(%) | 4.3% | 4.9% | 5.4% | 6.7% | 6.9% | 7.5% |
| Concentration(pg/ml) | 0 | 156 | 313 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.131 | 0.253 | 0.335 | 0.536 | 0.815 | 1.219 | 1.671 | 2.301 |
1：准备试剂和标准品
2：每孔添加100ul样品和标准品，37℃反应90分钟，不洗。
3：每孔添加100ul生物素标记抗体，37℃反应60分钟。
4：0.01M TBS洗涤3次。
5：每孔添加100ulABC，37℃反应30分钟。
6：0.01M TBS洗涤5次。
7：TMB37℃避光反应30分钟以内。
8：加入TMB终止液，读数。
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