货号 | EK1702 |
---|---|
产品名称 | Human Haptoglobin PicoKine ELISA Kit |
产品描述 | For quantitative detection of human Haptoglobin in cell culture supernates, serum and plasma (heparin, EDTA). |
免疫原 | NSO,V19-N406 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <20pg/ml |
检测范围 | 3.12ng/ml-200ng/ml |
基因名称 | HP |
基因全名 | haptoglobin |
Uniprot ID | P00738 |
指标别名 | Haptoglobin; Zonulin; Haptoglobin alpha chain; Haptoglobin beta chain; HP |
背景资料 | Haptoglobin(HP), is a protein that in humans is encoded by the HP gene. Haptoglobin, a plasma glycoprotein that binds free hemoglobin, has a tetrameric structure of 2 alpha and 2 beta polypeptides that are covalently associated by disulfide bonds. Haptoglobin is homologous to serine proteases of the chymotrypsinogen family. In the chimpanzee, there are 3 genes in the haptoglobin family(haptoglobin, HP; haptoglobin-related, HPR; and haptoglobin-primate, HPP), whereas only 2 genes exist in humans(HP and HPR). There are similarities between the primary structures of the alpha chain and of light chains of gamma globulins. The alpha haptoglobin locus is on the long arm of chromosome 16, where its exact cytogenetic location is 16q22.2. A major function of haptoglobin is to bind hemoglobin(Hb) to form a stable Hp-Hb complex and thereby prevent Hb-induced oxidative tissue damage. Haptoglobin is an unusual secretory protein in that it is proteolytically processed in the endoplasmic reticulum and not in the Golgi. The human haptoglobin HP*2 allele contains a 1.7-kb intragenic duplication that arose after a unique nonhomologous recombination between the prototype HP*1 alleles. |
显色时间 | 20-25 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK1702-CAP | 96-well plate precoated with anti- human Haptoglobin antibody | 1 |
EK1702-ST | Lyophilized recombinant human Haptoglobin standard | 200ng/tubex2 |
EK1702-DA | Biotinylated anti- human Haptoglobin antibody | 130ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 5711 | 36664 | 105148 | 5766 | 37146 | 11013 |
Standard deviation | 291.26 | 1979.86 | 4521.36 | 282.53 | 2488.78 | 870.03 |
CV(%) | 5.1% | 5.4% | 4.3% | 4.9% | 6.7% | 7.9% |
Concentration(ng/ml) | 0 | 3.12 | 6.25 | 12.5 | 25 | 50 | 100 | 200 |
O.D. | 0.070 | 0.332 | 0.526 | 0.998 | 1.329 | 1.720 | 1.961 | 2.389 |
1:准备试剂和标准品
2:每孔添加100ul样品和标准品,37℃反应90分钟,不洗。
3:每孔添加100ul生物素标记抗体,37℃反应60分钟。
4:0.01M TBS洗涤3次。
5:每孔添加100ulABC,37℃反应30分钟。
6:0.01M TBS洗涤5次。
7:TMB37℃避光反应30分钟以内。
8:加入TMB终止液,读数。
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